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Image Search Results
Journal: Genes & Development
Article Title: 3′ end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells
doi: 10.1101/gad.231274.113
Figure Lengend Snippet: ( A ) Diagram of the human MYC gene with exons (black boxes) and the pA site indicated. Regions assayed by ChIP are underlined and labeled, and a scale marker is shown. ( B ) ChIP experiment assaying the distribution of Pol II (N20), Ser5p, Ser2p, and Ser7p across the MYC gene. For each antibody, values are normalized to the maximum value (see the Materials and Methods for details). (NTS) A negative control region. ( C ) ChIP experiment assaying the distribution of CPSF30 and CstF77 across the MYC gene. For each antibody, values are normalized to the maximum value. (NTS) A negative control region. ( D ) Native ChIP analyzing Ser2p across the MYC gene. Values are normalized to the maximum value. (NTS) A negative control region. All error bars represent standard deviation from at least three biological replicates.
Article Snippet: Antibodies used were Pol II (N20:sc899, Santa Cruz Biotechnology), Ser2p (3E10, ChromoTek), Ser5p (3E8, ChromoTek), Ser7p (4E12, ChromoTek), CstF77 (C0249, Sigma), CPSF73 (C2747, Sigma),
Techniques: Labeling, Marker, Negative Control, Standard Deviation